All of the clinically prevalent carbapenemase, ESBL and AmpC genes in a single microarray.

1. Identification

• The probe arms will be ligated together only if they match exactly to the template.
• A single nucleotide mismatch will prevent ligation of the probe arms.

2. Amplification

• Only ligated probes are amplified (and labeled).

3. Detection

• Different probes will hybridize to different positions on the microarray through the ZIP region of the probe.
• Hybridized amplification products are visualized by colorimetric detection.

4. Analysis

• An image of the microarray, contained in an Array Tube, is generated using the Check-Points Tube Reader.
• The E-Ads software automatically analyzes the presence or absence of carbapenemases, AmpCs and ESBLs as well as the resistance genes present.