Polymerase Chain Reaction, PCR, was invented in 1985 and was awarded the Nobel Prize for chemistry in 1993. To date, more than thirty years after its initial conception, it is still the most sensitive method for detection of specific DNA sequences in biological specimens. An important addition to PCR was the invention of real-time PCR employing multiple specific fluorescent DNA probes to detect the accumulation of amplification products. Real-time PCR is the method of choice for sensitive detection of pathogens in clinical specimens.
Therefore, Check-Points has chosen this technology for clinical applications where a very high sensitivity is required. Check-Points has developed three multiplex real-time PCR tests for detection of multidrug resistance (MDR) in gram-negative bacteria: BD MAX Check-Points CPO, Check-Direct ESBL Screen for BD MAX and the Check-Direct CPE for a number of specific PCR machines.